Deoxyribonucleic acid sodium salt from salmon testes
Deoxyribonucleic acid sodium salt from salmon testes
CAS: 438545-06-3
Molecular Formula:
Deoxyribonucleic acid sodium salt from salmon testes - Names and Identifiers
| Name | Deoxyribonucleic acid sodium salt from salmon testes |
| Synonyms | smDNA DNA SS-DNA Deoxyribonucleic Acid Sodium Salt from Deoxyribonucleic acid sodium salt from salmon testes |
| CAS | 438545-06-3 |
Deoxyribonucleic acid sodium salt from salmon testes - Physico-chemical Properties
| Storage Condition | 2-8°C |
Deoxyribonucleic acid sodium salt from salmon testes - Risk and Safety
| WGK Germany | 3 |
Deoxyribonucleic acid sodium salt from salmon testes - Uses and synthesis methods
overview
salmon essence DNA solution is a single-stranded DNA solution of short segments after phenol chloroform extraction, ultrasonic and thermal denaturation treatment, which can be directly used in nucleic acid hybridization such as Southern and Northern. The concentration of salmon sperm DNA of this product is 10 mg/mL, which can be diluted to the required working concentration according to the specific requirements of the experiment.
Salmon Sperm DNA(438545-06-3,Deoxyribonucleic Acid Sodium Salt from salmon testes) is used for anaerobic growth of several species of Bacillus mori in basal medium, Salmon Sperm DNA is also used as the main component of the microplate-based matrix chromatin immunoprecipitation (IP) buffer for immunoprecipitation.
Salmon Sperm DNA is also known as deoxyribonucleic acid (SS-DNA). This product can be used to study the physical and chemical interactions of DNA with binding agents, modifiers, inserts, detection agents and dense agents. Salmon sperm DNA has also been applied to study magnetic mesopores, the loading capacity of silica nanoparticles (M-MSNs), and the binding capacity of polymer nanolayer structures. Salmon sperm DNA can also be used as a substrate for the development of DNA detection methods.
Absorption rate (0.001% solution in phosphate buffer, pH 7.0,260 nm):≥ 0.155.
preparation method
1. weigh 2 g of salmon essence DNA and place it in a 500 ml beaker, and add about 200 ml TE Buffer.
2. stir at room temperature with a magnetic stirrer for 2-4 hours, add 4 ml of 5 M NaCl after dissolution, and make the final concentration 0.1 M.
3. extract with phenol and phenol/chloroform once each.
4. After recovering the aqueous solution, use the No. 17 hypodermic needle to quickly suck the solution about 20 times to cut off the DNA.
5. Add 2 times the volume of precooled ethanol for ethanol precipitation.
6. after centrifugation to recover DNA, dissolve it in 100 ml of deionized water, and determine the OD260 value of the solution.
7. After calculating the DNA concentration of the solution, dilute the DNA solution to 10 mg/ml.
8. after boiling for 10 minutes, pack into small portions (1 ml/portion). -20 ℃.
9. After heating in a boiling water bath for 5 minutes before use, quickly cool in an ice bath.
Use common methods
Common problems in the use of salmon sperm DNA:
What is the molecular weight of the salmon testis (DNA) product deoxyribonucleic acid sodium salt?
The molecular weight of salmon essence has not been determined. In general, references J.A.C.S.,vol. 118,10679(1996) reported a molecular weight of 1.3 million or about 2,000 base pairs.
What is the solubility of the product deoxyribonucleic acid sodium salt in salmon sperm DNA?
Van der Bio tested its solubility in water to be 2 mg/mL. To prevent shear of large genomic DNA, the product should be dissolved in water (1 mg/mL) without ultrasonic treatment or stirring. Mild overnight at 0-4°C for complete DNA dissolution is recommended. 1 mM EDTA is recommended to prevent DNA degradation by nuclease.
What is the solution stability of the sodium deoxyribonucleic acid salt in salmon testis DNA?
Salmon essence DNA solution has been successfully stored for several months at 4°C,10 mM Tris,pH 7.5-8.0, 1 mM EDTA and no bacteriostatic.
Last Update：2024-04-10 22:29:15
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Spot supply
Product Name: DNA Na(Salmon sperm ) Visit Supplier Webpage Request for quotationCAS: 438545-06-3
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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